Proteomics

分析化學的應用範疇：何為蛋白體學檢測

Proteomics is a technique to rapidly identification of a large number of proteins in biofluids, tissues or any extracted solutions. Proteomics

can be used for biomarker study, mechanism elucidation, protein-drug interaction, bacterial identification, food adulteration, etc.

技術原理

Proteins are digested into peptides. A sensitive nanoLC-MS/MS system is used to detect the complex peptides. The MS/MS datafiles are

searched against a protein database to identify proteins in the sample solution. In a single run of nanoLC-MS/MS of cellextracts,

thousands of proteins can be identified.

案例分享

・Prognostic tools and candidate drugs based on plasma proteomics of patients with severe COVID-19 complications

Al-Nesf, M.A.Y., Abdesselem, H.B., Bensmail, I. et al. Prognostic tools and candidate drugs based on plasma proteomics of patients with severe

COVID-19complications. Nat Commun 13, 946 (2022). https://doi.org/10.1038/s41467-022-28639-4

・Application of Proteomics in Cancer:Recent Trends and Approaches for Biomarkers Discovery

Yang Woo Kwon, Han-Seul Jo, Sungwon Bae, et al,Application of Proteomics in Cancer: Recent Trends and Approaches for Biomarkers Discovery

Front Med (Lausanne) . 2021 Sep 22:8:747333. doi:10.3389/fmed.2021.747333.

文獻

Selected Proteomics References

(1) Swietlik, J.J.; Barthel, S.; Falcomata, C.; Fink, D.; Sinha, A.; Cheng, J.; Ebner, S.; Landgraf, P.; Dieterich, D. C.; Daub, H.; et al. Cell-selective

proteomics segregates pancreatic cancer subtypes by extracellular proteins in tumors and circulation. Nat Commun 2023, 14 (1),2642.

(2) Marto, J. P.; Carvalho, A. S.; I, G. M.; Mendonca, M.; Salavisa, M.; Meira, B.; Fernandes, M.; Serrazina, F.; Cabral, G.; Ventura, R.; et al.

Proteomics to Identify New Blood Biomarkers for Diagnosing Patients With Acute Stroke. J Am Heart Assoc 2023, 12 (22), e030021.

(3) Cejnar, P.; Kuckova, S.; Santrucek, J.; Glasa, M.; Kominek, P.; Mihalik, D.; Slavikova, L.; Leisova-Svobodova, L.; Smirnova, T.; Hynek, R.; et al.

Efficient Confirmation of Plant Viral Proteins and Identification of Specific Viral Strains by nanoLC-ESI-Q-TOF Using Single-Leaf-Tissue Samples.

Pathogens 2020, 9 (11).

(4) Smirnova, T. A.; Viskin, A.; Hoskova, M.; Habartova, L.; Setnicka, V.; Cejnar, P.; Kuckova, S.Comparison of proteomic approaches used for the

detection of potential biomarkers of Alzheimer's disease in blood plasma. J Sep Sci 2021, 44 (22), 4132-4140.

(5) Meier, F.; Brunner, A. D.; Koch, S.; Koch, H.; Lubeck, M.; Krause, M.; Goedecke, N.; Decker, J.; Kosinski, T.; Park, M. A.; et al. Online Parallel

Accumulation-Serial Fragmentation (PASEF) with a Novel Trapped Ion Mobility Mass Spectrometer. Mol Cell Proteomics 2018, 17 (12), 2534-2545.

(6) Viode, A.; van Zalm, P.; Smolen, K. K.; Fatou, B.; Stevenson, D.; Jha, M.; Levy, O.; Steen, J.; Steen, H.; Network, I. A simple, time- and cost-effective,

high-throughput depletion strategy for deep plasma proteomics. Sci Adv 2023, 9 (13), eadf9717.

Q-TOF Orbitrap Exploris™ 480